The Effect of Giving Red Fruit Oil (Pandanus Conoideus Lam.) on Degeneration and Necrosis Levels of Mice Hepatocyte (Mus musculus) Exposed to Plumbum

Abstract

Plumbum exposure is known can induce excessive production of Reactive Oxygen Species (R.O.S.) and cause oxidative stress on the cell. Oxidative stress can cause disorders in the body's organs, one of which is the liver organ. The study aims to determine the effect of giving red fruit oil as a protection against the exposure of plumbum at the rate of degeneration and cell necrosis of the liver. Red fruit oil contains antioxidant compounds beta carotene and tocopherol that function as antidotes to free radicals to prevent oxidative stress. This study was conducted for 14 days with four treatments. Namely P0 (negative control), P1 (positive control given exposure to plumbum at a dose of 0, 01mg), P2 (0.3 ml oil of red Fruit and exposure to plumbum at a dose of 0, 01mg), and P3 (0.8 ml of red fruit oil and exposure to plumbum at a dose of 0, 01mg). Determination of the level of degeneration and necrosis of liver cells is done by looking at the hematoxylin-eosin (HE) preparation of mice's liver under the microscope with magnification 400x. This study showed that the red fruit oil at a dose of 0.3ml and 0.8ml increased the effect on degeneration and necrosis of a mice's liver cell exposure to plumbum.